Analytical Technologies in Biotechnology

Analytical Technologies in Biotechnology. Instructor: Dr. Ashwani K. Sharma, Department of Biotechnology, IIT Roorkee. Welcome to this course on analytical technologies in biotechnologies. It is quite evident that advances in technology has been vital in the progress of science. In life sciences, advances in analytical techniques have played a crucial role in understanding of biological functions at the molecular and the cellular label. Biotechnology is an interdisciplinary science that involves applications of chemical and physical techniques, in the field of life sciences. This course covers lessons on Microscopy, Radioisotope techniques, Chromatographic methods, Electrophoresis, Centrifugation techniques, Spectroscopic techniques, Polymerase Chain Reaction(PCR), DNA sequencing, and Enzyme linked immunosorbent assay. (from

Basic Concepts in Microscopy

Lecture 01 - Basic Concepts in Microscopy
Lecture 02 - Basic Concepts in Microscopy (cont.)
Lecture 03 - Dark-field and Phase Contrast Microscopy
Lecture 04 - Differential Interference Contrast and Polarization Microscopy
Lecture 05 - Fluorescence and Confocal Microscopy
Lecture 06 - Transmission Electron Microscopy
Lecture 07 - Transmission Electron Microscopy (cont.), Scanning Electron Microscopy
Radioisotopes Techniques
Lecture 08 - Basic Concepts
Lecture 09 - Basic Concepts (cont.)
Lecture 10 - GM Counting and Scintillation Counting
Lecture 11 - Scintillation Counting (cont.)
Lecture 12 - Autoradiography and RIA
Lecture 13 - Safety Aspects and Applications
Chromatographic Methods
Lecture 14 - Introduction and Basic Concepts in Chromatography
Lecture 15 - Basic Concepts in Chromatography
Lecture 16 - Low-Pressure Liquid Chromatography (LPLC) and High Performance Liquid Chromatography (HPLC)
Lecture 17 - Ion-exchange Chromatography
Lecture 18 - Gel Filtration Chromatography
Lecture 19 - Affinity Chromatography
Lecture 20 - Gas-Liquid Chromatography
Lecture 21 - Basis Concept in Electrophoresis
Lecture 22 - Horizontal and Vertical Gel Electrophoresis
Lecture 23 - Native Gel Electrophoresis and SDS-PAGE
Lecture 24 - Isoelectric Focusing, 2D Gel Electrophoresis and Protein Detection Methods
Lecture 25 - Electrophoresis of Nucleic Acids
Lecture 26 - Immunoelectrophoresis and Capillary Electrophoresis
Centrifugation Techniques
Lecture 27 - Introduction and Basic Concepts
Lecture 28 - Basic Concepts (cont.)
Lecture 29 - Types of Centrifuges and Analytical Ultracentrifugation Method
Lecture 30 - Separation Methods in Preparative Ultracentrifuges
Lecture 31 - Types of Rotors
Lecture 32 - Types of Rotors (cont.), Care of Rotors
Spectroscopic Techniques
Lecture 33 - Introduction and Basic Concepts
Lecture 34 - UV-Visible Spectroscopy
Lecture 35 - Infrared and Fluorescence Spectroscopy
Lecture 36 - Circular Dichroism (CD) Spectroscopy
Lecture 37 - Nuclear Magnetic Resonance (NMR) Spectroscopy and X-ray Crystallography
Lecture 38 - Atomic Spectroscopy and Mass Spectrometry
Polymerase Chain Reaction
Lecture 39 - Polymerase Chain Reaction (PCR)
Lecture 40 - DNA Sequencing Methods
Lecture 41 - Enzyme Linked Immunosorbent Assay (ELISA)

Analytical Technologies in Biotechnology
Instructor: Dr. Ashwani K. Sharma, Department of Biotechnology, IIT Roorkee. This course deals with topics in analytical technologies in biotechnologies.