Mass Spectrometry Based Proteomics

Mass Spectrometry Based Proteomics. Instructor: Prof. Sanjeeva Srivastava, Department of Biosciences and Bioengineering, IIT Bombay. Mass spectrometry is an advanced analytical technique for accurate mass measurement. It does so by producing charged molecular species in vacuum followed by its separation in the magnetic and electric fields on the basis of their mass to charge (m/z) ratio. MALDI and ESI coupled with mass analyzers are commonly used mass spectrometer configuration in proteomics. The human proteome draft was decoded by using high-resolution liquid chromatography coupled with mass spectrometry.

In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics. (from

Lecture 02 - Proteomics and Sample Preparation

Go to the Course Home or watch other lectures:

Proteomics Introduction and Sample Preparation
Lecture 01 - Introduction to Proteomics
Lecture 02 - Proteomics and Sample Preparation
Lecture 03 - Bacterial Protein Extraction
Lecture 04 - In-gel Digestion
Lecture 05 - Fundamentals of Mass Spectrometry
Basics of Mass Spectrometry
Lecture 06 - Chromatography Techniques
Lecture 07 - Liquid Chromatography
Lecture 08 - Mass Spectrometry: Ionization Sources
Lecture 09 - Mass Spectrometry: Mass Analyzers
Lecture 10 - MALDI Sample Preparation and Analysis
Quantitative Proteomics
Lecture 11 - Introduction to Quantitative Proteomics
Lecture 12 - Hybrid Mass Spectrometry Configurations
Lecture 13 - SILAC: In Vivo Labeling
Lecture 14 - iTRAQ: In Vivo Labeling
Lecture 15 - TMT: In Vivo Labeling
Proteomics and Systems Biology
Lecture 16 - Quantitative Proteomics Data Analysis
Lecture 17 - Proteomics and Systems Biology
Lecture 18 - Proteomics and Systems Biology (cont.)
Lecture 19 - Proteomics Applications
Lecture 20 - Advances and Challenges in Proteomics