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Principles and Applications of NMR Spectroscopy

Principles and Applications of NMR Spectroscopy. Instructor: Prof. Hanudatta S. Atreya, NMR Research Centre, IISc Bangalore. The objective of the course is to teach the basic aspects of nuclear magnetic resonance (NMR) spectroscopy, which is an important analytical tool in chemical and pharmaceutical industry for structural characterization of molecules. The topics to be covered will include one-dimensional NMR, Chemical shifts, J-coupling, Interpretation of 1D NMR spectrum, Basics of 2D NMR, Different 2D NMR experiments and their application/interpretation, Application of 2D NMR for assignment of molecules and peptides. (from nptel.ac.in)

Lecture 25 - 2D NOESY and 2D ROESY


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Instruction to NMR Spectroscopy
Lecture 01 - Instruction to NMR Spectroscopy
Lecture 02 - The Alignment of Nuclear Spins in Presence of Magnetic Field
Lecture 03 - Introduction to Rotating Frame
Lecture 04 - Free Induction Decay and Fourier Transformation of FID
Lecture 05 - NMR Hardware
Chemical Shifts and J-Coupling
Lecture 06 - The Concept of Chemical Shift
Lecture 07 - Factors that Affect Chemical Shifts
Lecture 08 - Chemical Shift Referencing
Lecture 09 - J-Coupling
Lecture 10 - Recap of Basics
One-Dimensional Proton NMR
Lecture 11 - Introduction to General One-Dimensional NMR Experiment
Lecture 12 - Practical Aspects of Recording a 1D NMR Experiment I
Lecture 13 - Practical Aspects of Recording a 1D NMR Experiment II
Lecture 14 - Practical Aspects of Recording a 1D NMR Experiment III
Lecture 15 - NMR Data Processing
One Dimensional NMR of X-nuclei (13C, 15N, 31P and 19F)
Lecture 16 - Basic Aspects of 1D Proton NMR Analysis
Lecture 17 - Analysis of an Example of 1D Proton Spectrum
Lecture 18 - Analysis of 1D 1H NMR Spectra of Molecules
Lecture 19 - Analysis of 1D 1H NMR Spectra of Molecules (cont.)
Lecture 20 - 1D 13C NMR
Homonuclear 2D NMR
Lecture 21 - Why do We Need 2D NMR?
Lecture 22 - A Qualitative Explanation of How 2D NMR Experiment Works
Lecture 23 - Principles of 2D COSY and Total Correlation Spectroscopy (2D TOCSY)
Lecture 24 - 2D NOE Spectroscopy
Lecture 25 - 2D NOESY and 2D ROESY
Heteronuclear 2D NMR
Lecture 26 - What is Heteronuclear Correlation NMR Spectroscopy?
Lecture 27 - Sensitivity Enhancement of Heternuclei via Polarization Transfer
Lecture 28 - Heteronuclear Multiple Quantum NMR Spectroscopy (2D HMQC) and Heteronuclear Single Quantum NMR Spectroscopy (2D HSQC)
Lecture 29 - Practical Aspects of Recording and Processing 2D HMQC or HSQC
Lecture 30 - HMQC and its Utility
Structure Determination of Molecules
Lecture 31 - 2D HSQC TOCSY and its Analysis with Examples
Lecture 32 - Structure Determination of Molecules by NMR
Lecture 33 - Structure Determination of Peptides I
Lecture 34 - Structure Determination of Peptides II
Lecture 35 - Structure Determination of Peptides III
Advanced Topics (Solvent Suppression, Drug Discovery, DOSY)
Lecture 36 - Chemical Exchange
Lecture 37 - Hydrogen or Deuterium Exchange
Lecture 38 - Diffusion Ordered Spectroscopy (DOSY)
Lecture 39 - Diffusion Ordered Spectroscopy (cont.)
Lecture 40 - STD NMR for Drug Target Interactions